科研動(dòng)態(tài)

禽網(wǎng)狀內(nèi)皮組織增生癥病毒（reticuloendotheliosis virus，REV）是一種常見的禽腫瘤性病毒，可誘發(fā)感染雞群免疫抑制，給我國養(yǎng)禽業(yè)帶來巨大經(jīng)濟(jì)損失。本試驗(yàn)運(yùn)用DF-1細(xì)胞接種方法，在山東省某父母代白羽肉種雞場(chǎng)雞群中分離到1株REV，將其命名為SD2101株。為掌握SD2101株基因組遺傳演化特征，設(shè)計(jì)合成6對(duì)引物，通過PCR擴(kuò)增獲得了SD2101株的前病毒全基因組序列，并與GenBank下載的參考毒株進(jìn)行了序列比對(duì)和遺傳進(jìn)化分析。結(jié)果顯示：SD2101株前病毒全基因組具有典型復(fù)制完整型逆轉(zhuǎn)錄病毒的基因組結(jié)構(gòu)，與2011年分離自江蘇省的雞源REV野毒株HA1101同源性最高；遺傳進(jìn)化分析發(fā)現(xiàn)，SD2101株與大部分國內(nèi)分離株處于同一進(jìn)化支上。為了解SD2101株的致病性，將分離毒株接種于1日齡SPF雞，通過體質(zhì)量、免疫器官指數(shù)和滅活疫苗免疫應(yīng)答等指標(biāo)進(jìn)行評(píng)價(jià)。結(jié)果顯示，SD2101株對(duì)1日齡SPF雞的生長和免疫功能具有明顯的抑制作用。綜上，與參考毒株相比，SD2101株基因組序列變異程度較小，具有一定致病作用，提示在生產(chǎn)中應(yīng)加強(qiáng)對(duì)REV的關(guān)注和監(jiān)測(cè)。本研究為REV的流行病學(xué)研究提供了信息資料。

Isolation and Identification of Avian Reticuloendotheliosis Virus SD2101 Strain from Shandong Province and Analysis on Its Whole Genome Sequence and Pathogenicity

Reticuloendotheliosis virus（REV），a common avian neoplastic virus，could induce immunosuppression in infected chickens and bring huge economic losses to poultry industry in China. In this study，a REV strain was isolated from chicken flocks in a parental farm in Shandong Province through inoculating DF-1 cells and named as SD2101. In order to investigate its genetic evolution characteristics，six pairs of primers were designed and synthesized to obtain the whole genome sequence of its provirus by using PCR amplification，followed by sequence alignment and analysis on genetic evolution with the reference strains downloaded from GenBank. The results showed that the provirus was with a typical genome structure of replication-competent retrovirus，with the highest homology with HA1101 which was isolated from chickens in Jiangsu Province in 2011. It was found that，by analysis on genetic evolution，SD2101 strain was on the same evolutionary clade as most domestic isolates. In order to identify the pathogenicity of SD2101 strain，the isolate was inoculated into one-day-old specific pathogen free（SPF）chickens，which was then evaluated by body weight，indexes of immune organs and immune response of inactivated vaccine. In conclusion，the growth and immune functions of one-day-old SPF chickens were significantly inhibited by SD2101 strain. Compared with the reference strain，a small degree of genomic sequence variation was observed in SD2101 strain，with certain pathogenicity. Scientific data was thus provided for further epidemiological research on REV，as it was suggested to focus on and monitor the virus in practice. 

原文鏈接：https://chn.oversea.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFD&dbname=CJFDAUTO&filename=ZGDW202206007&uniplatform=OVERSEAS_CHS&v=vPzqR5W2eDDCCa8x0eUlqRwe3KUFA0bYqdbrEhm8tVlQwrLXEqbGNtRqMXLlpanp