為觀察豬流行性腹瀉病毒（PDEV）的血凝特性，進(jìn)而為建立PEDV HA和HI試驗(yàn)，評估疫苗免疫抗體水平奠定基礎(chǔ)，將CV777、DR13、YN13和YN144共4株P(guān)EDV毒株，在37 ℃下，分別用0、5、10、50、100 μg/mL胰蛋白酶處理30 min，然后用雞、兔和豬紅細(xì)胞進(jìn)行血凝（HA）試驗(yàn)；用已經(jīng)鑒定為PEDV抗體陽性的血清和乳清分別進(jìn)行血凝抑制（HI）試驗(yàn)。結(jié)果顯示：4株P(guān)EDV毒株只與兔紅細(xì)胞發(fā)生凝集反應(yīng)；CV777、DR13毒株的HA現(xiàn)象需要用5 μg/mL的胰蛋白酶進(jìn)行預(yù)處理，但是YN13和YN144毒株的HA現(xiàn)象不需要胰蛋白酶作用；該凝集現(xiàn)象能夠被PEDV抗體特異性抑制。結(jié)果表明，PEDV能夠凝集兔的紅細(xì)胞，不能凝集雞和豬的紅細(xì)胞，因而可以用兔紅細(xì)胞建立PEDV的HA和HI試驗(yàn)方法。
HA-HI Test for Four Strains of Porcine Epidemic Diarrhea Virus
In order to observe the hemagglutination（HA）of porcine epidemic diarrhea virus（PDEV）and to lay a foundation for development of HA-HI test for PEDV and evaluation of the antibody level，four strains of PEDV，including CV777，DR13，YN13 and YN144，were treated with trypsin of 0，5，10，50 and 100 μg/mL respectively for 30 minutes at 37 ℃，followed by HA test by use of red blood cells（RBC）of chicken，rabbit and pig respectively；then hemagglutination inhibition（HI）test was carried out with serum and whey that had been identified to be PEDV-positive. The results showed that four strains of PEDV only agglutinated with rabbit RBC. After CV777 and DR13 strain were pretreated with 5 μg/mL trypsin，the HA phenomenon occurred，while no trypsin was needed for YN13 andYN144 strain. In addition，HA could be specifically inhibited by antibody against PEDV. In conclusion，rabbit RBC rather than chicken and swine RBC could be agglutinated by PEDV，therefore，the HA-HI test for PEDV could be developed by use of rabbit RBC.
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