鴨圓環(huán)病毒病是由鴨圓環(huán)病毒（duck circovirus，DuCV）引起的一種重要免疫抑制病，影響著我國(guó)養(yǎng)鴨業(yè)的發(fā)展。為建立針對(duì)DuCV的PCR快速檢測(cè)方法，對(duì)GenBank中登錄的DuCV全基因組序列進(jìn)行遺傳進(jìn)化分析，通過(guò)對(duì)兩種基因型的DuCV全基因組序列進(jìn)行比對(duì)，在共同保守區(qū)篩選出1對(duì)引物，建立了可以同時(shí)檢測(cè)DuCV兩種基因型的PCR快速檢測(cè)方法，并對(duì)該方法的敏感性和特異性進(jìn)行評(píng)價(jià)。結(jié)果顯示，該方法具有良好的敏感性，檢測(cè)下限達(dá)1.0 fg；特異性良好，對(duì)其他常見(jiàn)鴨病毒核酸檢測(cè)均為陰性；利用該方法對(duì)20份臨床發(fā)病鴨樣品進(jìn)行檢測(cè)，發(fā)現(xiàn)有6份呈DuCV陽(yáng)性，序列分析表明均屬于DuCV-1。該P(yáng)CR檢測(cè)方法的建立為鴨圓環(huán)病毒病的快速診斷和防控提供了重要手段。
Development and Application of a PCR Assay for Duck Circovirus
Duck circovirus（DuCV）disease is a serious immunosuppressive disease caused by DuCV，posing a threat to the duck farming in China. In order to establish a rapid PCR assay for DuCV，the genetic evolution analysis was performed for the whole genome sequence of two DuCV genotypes that were registered in GenBank and subsequently compared with each other，then a pair of primers were screened in their common conservative area to establish a rapid PCR assay that could detect the two genotypes simultaneously. Then the sensitivity and specificity of the method were evaluated. The results showed that，by the method，the lower detection limit could reach 1.0 fg due to its good sensitivity；all nucleic acid detection for other common duck viruses were negative due to its good specificity；6 out of 20 samples of clinically infected ducks were positive for DuCV，which fell into DUCV-1 as indicated by sequence analysis. In conclusion，the method could rapidly diagnose，prevent and control DuCV as an important tool.
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