科研動態(tài)

為驗證豬繁殖與呼吸綜合征（PRRS）嵌合病毒活疫苗（PC株）和滅活疫苗聯(lián)合應(yīng)用于母豬的免疫效果，在天津市某PRRS陽性豬場，對部分母豬實施了兩種疫苗聯(lián)合免疫試驗，利用RT-PCR和間接ELISA方法進行病毒和免疫抗體檢測。結(jié)果顯示：接種疫苗后，試驗?zāi)肛i精神、食欲和體溫均正常，豬肛拭子和飼養(yǎng)環(huán)境樣品中均未檢測到病毒；免疫后14~70 d，試驗?zāi)肛iPRRS抗體陽性率均為100%；免疫后42 d，試驗?zāi)肛i的抗體IRPC平均值仍維持在較高水平，且免疫后14 d，2個試驗組變異系數(shù)分別下降31和44個百分點；試驗?zāi)肛i所產(chǎn)仔豬的均勻度、活潑程度均高于對照組。結(jié)果表明，PRRS嵌合病毒活疫苗（PC株）和滅活苗聯(lián)合免疫安全有效，不僅可以很好地誘導(dǎo)機體產(chǎn)生正常的免疫反應(yīng)，而且還可以提高豬群抗體的整齊度且無排毒風(fēng)險，免疫保護期較長。本研究為陽性豬場的PRRS防控提供了有效免疫方案。

Application Test of Combined Vaccination of Live Chimeric Virus Vaccine（PRRSV PC Strain）and Inactivated Vaccine in Sows

In order to verify the immune effect of combined vaccination of chimeric virus vaccine（PC strain）and inactivated vaccine of porcine reproductive and respiratory syndrome（PRRS）applied in sows，some sows in a PRRS positive farm in Tianjin City were simultaneously vaccinated with the two kinds of vaccines，and related samples were collected and detected for virus nucleic acids and antibodies by RT-PCR and indirect ELISA，respectively. The results showed that the experimental sows were with normal spirit，appetite and body temperature，and no virus was detected in their samples of anal swabs and feeding environment；the positive rate was 100% in 14 to 70 days after vaccination；the average IRPC of antibodies remained at a relatively high level in 42 days after vaccination，and the variation coefficient of the two experimental groups decreased by 31% and 44% respectively in 14 days after vaccination；the evenness and liveliness of piglets produced by the experimental sows were higher than those by control group. In conclusion，combined vaccination of chimeric virus vaccine（PC strain）and inactivated vaccine of PRRS was safe and effective，which not only induced normal immune response in the body，but also improved the uniformity of antibodies in pigs without any risk of detoxification，and provided a long immune protection period. An alternative and effective choice for PRRS prevention and control was thereby provided for PRRS-positive farms.  

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