為了解福建省雞場圓圈病毒3型（GyV3）的流行及其VP2基因變異情況，2021年在南平市、龍巖市和福州市的36個雞場，采集365份疑似GyV3感染病雞的腺胃樣品，通過PCR進(jìn)行GyV3核酸檢測。結(jié)果顯示：共檢出GyV3核酸陽性55份，陽性檢出率為15.06%。對5份GyV3陽性樣品進(jìn)行VP2基因序列分析發(fā)現(xiàn)，5份樣品的VP2基因核苷酸和推導(dǎo)氨基酸序列相似性分別為98.6%~99.9%和98.3%~100%，與參考株山東肉雞分離株SDAU-1和巴西家雞分離株核苷酸和推導(dǎo)氨基酸序列的相似性分別為98.3%~99.2%和97.5%~99.2%，均屬于Group A分支中的GyV3小分支。結(jié)果表明：福建省雞場存在GyV3流行；其VP2基因遺傳序列穩(wěn)定，可作為GyV3感染流行病學(xué)監(jiān)測的首選基因。本研究為GyV3的后續(xù)研究提供了理論依據(jù)。
Etiological Detection of GyV3 in Some Farms of Fujian Province and Analysis on Its VP2 Gene
In order to investigate the prevalence and VP2 gene variation of gyrovirus 3（GyV3）in chicken farms in Fujian Province in 2021，365 suspected samples of glandular stomach infected with GyV3 were collected from 36 farms in Nanping，Longyan and Fuzhou cities to detect GyV3 nucleic acid by PCR. The results showed that 55 positive samples were detected，with a positive rate of 15.06%. It was found that，by sequence analysis of VP2 gene in 5 GyV3 positive samples，their homology of nucleotide and deduced amino acid sequence of VP2 gene were from 98.6% to 99.9% and 98.3% to 100%，respectively，from 98.3% to 99.2% and 97.5% to 99.2% with those of the reference Shandong broiler strain SDAU-1 and Brazil domestic chicken strain，respectively，all of which belonged to the small branch of GyV3 in group A. In conclusion，GyV3 was prevalent in the farms across the province，and stable genetic sequence was observed in its VP2 gene，which could be used as the first choice for epidemiological surveillance over GyV3 infection. A theoretical basis was thereby provided for future researches on GyV3.
信息來源：https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&dbname=CJFDAUTODAY&filename=ZGDW202208003&uniplatform=NZKPT&v=vPzqR5W2eDACMu4nN2FrbQUNCucSQCAXG47vSQCRm6GDYHaWc6NGTJAAmXG6Wq6u
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